• Job Type: Support Staff - Union
• Bargaining Unit: SSA
• Regular/Temporary: Regular
• End Date if Temporary: n/a
• Hours Per Week: 35
• Salary Range: $1,290.15 per week

Position Summary

Responsibilities

• Isolate tissues and stain for phenotyping for postdoctoral researchers
• Maintain tissue culture room supplies needed to process tissues
• Perform genotyping and phenotyping for entire swine colony
• Process a variety of samples including blood, bone marrow, gut biopsies, or stoma pieces for PBMCs, IELs, and LPL
• Thaw healthy control samples for using in staining panels
• Stain processed samples with 18 colour flow cytometry antibody panels
• Freeze biopsy pieces, or single cell suspensions
• Update FreezerPro with Frozen samples
• Titrate Human Group Antibodies
• Participate in on call activities for human organ transplantation samples
• Perform weekly PBMC isolation from whole blood or DNA isolation
• Perform single color flow cytometry staining for SLA typing
• Responsible for freezing vials of PBMCs/Updating FreezerPro with PBMC locations
• Trouble shoot Swine SLA genotyping
• Perform single color flow cytometry FlowJo staining analysis
• Create the weekly typing report in Power Point to send for analysis
• Perform fibroblast culturing
• Order and maintain pig project supplies
• Make Facs buffer, heat inactivating sera, and perform sterile filtering
• Perform additional related duties as assigned

Minimum Qualifications

• Master's degree and at least three years experience or Bachelor's degree and at least five years of related experience, or equivalent in education, training and experience
• Ability to communicate with and train other lab staff

Preferred Qualifications

• Experience with sophisticated cellular assays, FACS sorting, DNA extraction, reagent titration, high throughput sequencing and analysis of large data sets

Other Requirements

• The candidate selected for this position is required to undergo a drug screening test prior to a final offer of employment being made
• Work with laboratory animals
• Potential bloodborne pathogen exposure
• Participation in the medical surveillance program
• Successful completion of applicable compliance and system training requirements

Equal Opportunity Employer / Disability / Veteran

Columbia University is committed to the hiring of qualified local residents.