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University Park, Pennsylvania
Vancouver, British Columbia, Canada
University Park, Pennsylvania
San Antonio, Texas
Rootstown, Ohio
LaBelle, Florida
Posted: 17-Aug-22
Location: La Jolla, California
Salary: Open
Internal Number: 118294
UCSD Layoff from Career Appointment: Apply by 08/16/2022 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor.
Special Selection Applicants: 08/30/2022. Eligible Special Selection clients should contact their Disability Counselor for assistance.
This position will remain open until a successful candidate has been identified.
DESCRIPTION
The Department of Medicine is the largest department within the UCSD School of Medicine and is responsible for fulfilling the teaching, research, and clinical missions of the University of California, San Diego School of Medicine.
Under supervision from the Principal Investigator, the Research Assistant will conduct the following experiments with guided research in several different areas.
Isolation of vascular endothelial/smooth muscle cells from mouse heart and lung.Molecular biological experiments (i.e., cloning, development and application of Adenoviral- and Adeno-associated viral vectors Western Blot, immunoprecipitation, site-directed mutagenesis, PCR, qPCR). Electrophysiological experiments (i.e., measurement of Ca2+ and ROS concentration in cytoplasm and mitochondria). Immunofluorescence experiment. Perform data acquisition and analysis, grant and manuscript preparation, and presentation of findings at weekly lab meetings. Maintaining transgenic mice colony including breeding and genotyping, management of lab research records including methodological approaches, experimental records and protocols, and maintaining a clean lab and the function of lab equipment.
MINIMUM QUALIFICATIONS
Theoretical and working knowledge in advanced molecular biology and cell physiology.
Knowledge of aseptic techniques for cell culture.
Proven ability to contribute to experimental design, analysis interpretation of the results, and troubleshooting.
Experience assisting with writing scientific manuscripts and contributing to teamwork on in vitro cell electrophysiological experiments.
Ability to isolate animal cells and conduct molecular biological and cell physiological experiments.
Proven ability and experience in gene cloning, overexpression, and silencing in the cells and tissues.
Demonstrated ability in Western Blot, PCR, and qPCR.
Ability and experience in immunofluorescence experiments using fluorescence microscope.
Demonstrated skill to work with animals, as well as animal and human tissues.
Knowledge of the following specialized programs; GraphPad, EndNote, and MS Word/Excel/PowerPoint.
Effective and diplomatic oral and written communication and interpersonal skills. Ability to work with a variety of personnel from different laboratories and instruct in optimal procedures for assays.
SPECIAL CONDITIONS
- Employment is subject to a criminal background check.
- Must be available to work some weekends & evenings to complete experiments.
- Must be able to work with laboratory animals (mice).
Pay Transparency Act Full Salary Range: $51,949 - $61,972 Annually
The University of California, San Diego is required to provide a reasonable estimate of the compensation range for this role. This range takes into account the wide range of factors that are considered in making compensation decisions including but not limited to experience, skills, knowledge, abilities, education, licensure and certifications, and other business and organizational needs. It is not typical for an individual to be offered a salary at or near the top of the range for a position. Salary offers are determined based on final candidate qualifications and experience. The full salary range for this position is defined as the Full Pay Range. The budgeted salary or hourly range that the University reasonably expects to pay for this position defined as the Expected Pay Scale. Placement within the Expected Pay Scale is determined by internal equity, relevant qualifications, and collective bargaining agreements (when applicable).
