DESCRIPTION

The Department of Medicine (DOM) is the largest department within the UCSD School of Medicine and is responsible for fulfilling the teaching, research and clinical missions of the University of California, San Diego School of Medicine. The department employs approximately 500 salaried faculty, 168 academic appointees, 331 residents, fellows and postdocs and 480 staff personnel.

The cardiovascular research laboratory in the Department of Medicine at the University of California, San Diego, seeks a Staff Research Associate 3 (SRA3) who is highly motivated, creative, and devoted to the project goals. One position in cardiovascular physiology is immediately available. Our research focuses on endothelial dysfunction in the heart and lung during cardiovascular diseases. The incumbent will design and implement research protocols and conduct experiments for ongoing research projects in the laboratory. Duties and Responsibilities are 1) perform physiological experiments in mice, including evaluation of cardiac function using Millar system (e.g., RVSP, LVSP, dP/dt, EF), measurement of pulmonary and coronary flow using an ultrasound system, and isometric tension measurements in mouse distal pulmonary and coronary arteries using a wire myograph, 2) conducting molecular biological experiments (e.g., Western Blot, immunoprecipitation, PCR, qPCR, gene cloning), 3) electrophysiological experiments to measure Ca2+ and ROS concentration in cytoplasm and mitochondria and assess GAP junction intercellular communication, 4) immunofluorescence experiment, and 5) isolation of vascular endothelial/smooth muscle cells from mouse lungs and hearts. The incumbent will perform data acquisition and analysis, grant and manuscript preparation, and presentation of findings at weekly lab meetings. Additional responsibilities include maintaining transgenic mice colonies (i.e., breeding and genotyping), managing lab research records (e.g., methodological approaches, experimental records, and protocols), and maintaining a clean lab and the function of lab equipment. Aseptic techniques for tissue culture and animal handling are mandatory skills in our laboratory.

MINIMUM QUALIFICATIONS

• Theoretical and working knowledge in advanced molecular biology and cell physiology.

• Aseptic techniques for cell culture.

• Demonstrated ability to contribute to experimental design, analysis, interpretation of the results, and troubleshooting.

• Demonstrated experience assisting with writing scientific manuscripts and contributing to teamwork in molecular biological experiments.

• Demonstrated ability and experience to isolate heart and lung vascular endothelial and smooth muscle cells and prepare primary cultured cells for molecular biological and cell physiological experiments.

• Demonstrated ability and experience in Western Blot, immunoprecipitation, site-directed mutagenesis, PCR, qPCR, and single-cell RT-PCR.

• Demonstrated ability and experience in electrophysiological experiments and immunofluorescence experiments using a fluorescence microscope.

• Demonstrated skill to work with animals, as well as animal and human tissues.

• Demonstrated knowledge of the following specialized programs; SnapGene, GraphPad, EndNote, and MS Word/Excel/PowerPoint.

• Demonstrated ability to read scientific literature, understand appropriate study design, follow study procedures and protocol, and maintain participant confidentiality.

• Demonstrated effective time management skills to plan and organize work assignments in an efficient and productive manner.

• Enthusiasm for learning and a genuine interest in scientific research. Willingness and ability to learn new skills, research methods, and scientific principles.

• Effective and diplomatic oral and written communication and interpersonal skills. Ability to work with a variety of personnel from different laboratories and instruct in optimal procedures for assays.

• Perform physiological experiments in mice, including evaluation of cardiac function using Millar system (e.g., RVSP, LVSP, dP/dt, EF), measurement of pulmonary and coronary flow using an ultrasound system, and isometric tension measurements in mouse distal pulmonary and coronary arteries using a wire myograph.

• Conduct electrophysiological experiments to measure Ca2+ and ROS concentration in cytoplasm/ER/mitochondria, and Gap junct

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  UC San Diego is an academic powerhouse and economic engine, recognized as one of the top 8 public universities by U.S. News and World Report. Innovation is central to who we are and what we do. Here, students learn that knowledge isn't just acquired in the classroom - life is their laboratory. UC San Diego's rich academic portfolio includes six undergraduate colleges, five academic divisions and five graduate and professional schools. The university's award-winning scholars are experts at the forefront of their fields with an impressive track record for achieving scientific, medical and technological breakthroughs.

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