The Department of Pediatrics is seeking a full time Research Technologist to work in a laboratory in the Division of Neonatal-Perinatal Medicine that focuses on neonatal hypoxic- ischemic brain injury. Consistent with a translational research program, our goal is to increase understanding of neonatal brain injury, repair and recovery mechanisms to identify new therapeutic targets and treatment strategies. The successful candidate will be able to work on multiple research projects simultaneously, while conducting experiments, collecting/summarizing data, providing proper laboratory/study subject care, maintaining project data, contributing to manuscripts and funding proposals with supervision and interfacing with a diverse collaborative of physicians and scientists.
Specific Duties & Responsibilities
Work requires basic use of scientific methods, procedures and techniques gained through previous training and experience.
Working knowledge of theory behind experiments and applies basic analytical skills to implement assigned projects, and to identify and trouble-shoot situations needing special attention.
Follow established procedures or protocols and utilizes aseptic technique to perform laboratory tests and/or experiments which may include basic tissue culture, cell separation methods, electrophoretic separations, assays, basic molecular biology, or other specialized histology and biological science techniques, including microscopy and biochemistry.
Utilize in vivo models in experiments and handle rats and mice.
Collect data and monitor data quality and accuracy as required by research protocol.
Maintain appropriate documentation of research results, as required by research protocol.
Recommend revisions to experimental methods based upon observations of the data.
Participate in experimental design; plan, implement, evaluate and interpret results.
Conduct library research; participate in editing and preparing manuscripts, scientific illustrations and computer graphics for publication.
Identify problems, troubleshoot, analyze variations observed and reported in regular testing protocols.
Modify, refine, adapt techniques/procedures.
Perform surgeries and care for laboratory animals; perform behavioral testing.
Follow experimental protocols, collect tissue in accordance with USDA, AALAC and IACUC guidelines.
Monitor inventory levels, order materials and supplies in accordance with established policies and procedures, verify orders upon receipt.
Perform microscopy, flow cytometry and routine cell culture- after appropriate training.
Responsible for preventative maintenance of equipment.
Prepare, clean, sterilize, and maintain lab equipment, glassware, & instruments.
Assist with general lab and equipment maintenance.
Keep daily lab notes, maintain data and inventory spreadsheets.
Assist with laboratory compliance with regulatory requirements and JHU standards, policies/procedures.
Clean and maintain work areas.
Use computers and computer-interfaced equipment to perform required duties.
Analyze experimental data as appropriate.
Mentor students, teach laboratory techniques and share scientific knowledge.
Review, edit and maintain updated animal protocols.
Special Knowledge, Skills, & Abilities
Must be knowledgeable of skills required in immunoblotting, tissue culture, flow cytometry and microscopy and perform animal work independently. The candidate will perform established laboratory assays with the initial supervision of senior personnel until capable of performing those assays independently.
Self-motivated and actively seeking to further develop skills, expertise and knowledge in basic science and medicine.
Additional relevant laboratory experience acquired through extracurricular activities or summer employment is very desirable.
Molecular and Cellular Biology is highly desirable.
Working knowledge of basic lab equipment such as: centrifuge, pH Meter, analytical balance, incubator, spectrophotometer, light microscope and/or utilization of laminar flow hood, and other equipment as needed.
Detail oriented and conscientious; with attention to optimal levels of quantity and quality.
Excellent oral and written communication skills.
Ability to work independently, as well as in a group/team setting.
Ability to complete duties and responsibilities with limited supervision after training on new techniques.
Ability to make decisions and establish work priorities on procedure-oriented operations at the direction of faculty/PI and/or lab manager.
Working knowledge of computer programs (Excel, PowerPoint, statistical programs for basic analysis etc.); knowledge of policies, procedures and standard laboratory practices necessary to conduct the normal function of the lab.
Minimum Qualifications
Bachelor's Degree in biology, chemistry or related field.
Some related post-undergraduate work experience preferred.
Preferred Qualifications
Must have a working knowledge of one or more of the following disciplines achieved through undergraduate studies, and laboratory experience gained through class work: biology, biochemistry, chemistry, physics, microbiology and/or genetics.
Must have strong working knowledge of basic lab equipment and computer operations.
Some post-undergraduate experience in a comparable laboratory, or a significant independent research project
Undergraduate research experience as part of their training is acceptable experience.
Physical Requirements
Work environment may include areas of unpleasant extremes of cold or heat.
Biohazardous conditions such as the risk of radiation exposure, fumes or airborne particles, and/or toxic or caustic chemicals may be present in this work environment which mandate attention to safety procedures.
Near vision to see objects clearly within 20 inches.
Full spectrum vision to identify and distinguish color.
Finger dexterity required to manipulate objects with fingers rather than with whole hand(s) or arm(s), for example, using a keyboard.
Handling by seizing, holding, grasping, turning or otherwise working with the hand or hands.
Sitting or standing in a normal position for extended periods of time.
Occasional lifting, carrying objects weighing 10 lbs. or less.
Occasionally pushing, pulling objects weighing 30 lbs. or less.
Classified Title: Research Technologist Role/Level/Range: ACRO40/E/02/CC Starting Salary Range: $15.00-$20.40-$25.50 HRLY (Commensurate with experience) Employee group: Full Time Schedule: M – F; 8:30am – 5:00pm; some evenings/weekends may be necessary; candidate will need to be flexible to adjust schedule for animal care; some work weeks may require Tuesday – Saturday schedule. Exempt Status: Non-Exempt Location: School of Medicine Campus Department name: SOM Ped NICU Personnel area: School of Medicine
Total Rewards The referenced salary range is based on Johns Hopkins University's good faith belief at the time of posting. Actual compensation may vary based on factors such as geographic location, work experience, market conditions, education/training and skill level. Johns Hopkins offers a total rewards package that supports our employees' health, life, career and retirement. More information can be found here: https://hr.jhu.edu/benefits-worklife/
Please refer to the job description above to see which forms of equivalency are permitted for this position. If permitted, equivalencies will follow these guidelines: JHU Equivalency Formula: 30 undergraduate degree credits (semester hours) or 18 graduate degree credits may substitute for one year of experience. Additional related experience may substitute for required education on the same basis. For jobs where equivalency is permitted, up to two years of non-related college course work may be applied towards the total minimum education/experience required for the respective job.
**Applicants who do not meet the posted requirements but are completing their final academic semester/quarter will be considered eligible for employment and may be asked to provide additional information confirming their academic completion date.
The successful candidate(s) for this position will be subject to a pre-employment background check. Johns Hopkins is committed to hiring individuals with a justice-involved background, consistent with applicable policies and current practice. A prior criminal history does not automatically preclude candidates from employment at Johns Hopkins University. In accordance with applicable law, the university will review, on an individual basis, the date of a candidate's conviction, the nature of the conviction and how the conviction relates to an essential job-related qualification or function.
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All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or status as a protected veteran.
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The pre-employment physical for positions in clinical areas, laboratories, working with research subjects, or involving community contact requires documentation of immune status against Rubella (German measles), Rubeola (Measles), Mumps, Varicella (chickenpox), Hepatitis B and documentation of having received the Tdap (Tetanus, diphtheria, pertussis) vaccination. This may include documentation of having two (2) MMR vaccines; two (2) Varicella vaccines; or antibody status to these diseases from laboratory testing. Blood tests for immunities to these diseases are ordinarily included in the pre-employment physical exam except for those employees who provide results of blood tests or immunization documentation from their own health care providers. Any vaccinations required for these diseases will be given at no cost in our Occupational Health office.
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