About the Job:
80%. Conduct, oversee, and analyze data from multiple experiments, including:
- Isolation and culture of primary murine T-cells, including Regulatory T-cells
- Retroviral transduction and generation of chimeric antigen receptor T-cells/Regulatory
T-cells
- Conduct in vitro and in vivo functional assays to assess chimeric antigen receptor T-
cell/Regulatory T-cell function
- Phenotypic and functional assessment of cultured/modified T cells by flow cytometry
- Culture and maintain stocks of primary murine tumor cells lines
- Management of experimental animals and injection/ harvest of
tissues from animal models
- Teach and supervise culture techniques
- Accurately records experiments and results
- Routine lab maintenance
20%. Lab management and maintenance, including waste removal, supply inventory, as
well as other day-to-day laboratory cleanliness activities

All required qualifications must be included in the application materials

Required Qualifications:
BA/BS with major in biological science plus one year of experience, or equivalent combination education and experience to total five years.
- Experience with routine lab tasks

Preferred Qualifications:
Prior experience working with laboratory animals (preferably mice)
- Prior experience in the field of Immunology
- Prior experience with purification of primary cells (mouse or human)
- Flow cytometry experience, including antibody staining, compensation and data acquisition
- Proficiency with cell counting utilizing a hemacytometer
- Strong cell culture experience, including tumor lines and primary mouse cells
- Prior experience maintaining accurate documentation of procedures and experimental data from projects in logical and organized manner
- Experience reporting effectively on the progress and direction of individual projects
- Ability to initiate and process multiple projects simultaneously

About University of Minnesota Twin Cities

The University of Minnesota, founded in the belief that all people are enriched by understanding, is dedicated to the advancement of learning and the search for truth; to the sharing of this knowledge through education for a diverse community; and to the application of this knowledge to benefit the people of the state, the nation, and the world.

Connections working at University of Minnesota Twin Cities