This role will work in the Institute on the Biology of Aging and Metabolism (iBAM) in our Mouse Core. This role will contribute to mouse colony organization and maintenance, using our laboratoryâ™s mouse cloud database to create specimen labels, archiving biological samples, supply ordering, molecular biology techniques including immunoblotting and qRT-PCR, animal necropsies, tissue preservation, shipment of biological samples and recording healthspan data in the database. Continuing self-motivated education in aging research is expected.
 

Essential and other Functions:

50% Laboratory Research
Assist in coordinating animal tissue dissections for efficient sample collection for experimental needs. Animal husbandry, mouse colony management, collect and record data collected on live animals, mouse necropsies, collection and preservation of biological samples, performs IVIS imaging, dosing mice for drug studies, communicates with RAR veterinary staff concerning animal health cases, facilitates animal transfers, monitors health of experimental animals.

25% Sample and Data Management
With supervised oversight, use the labâ™s unique colony management database to create animal identifiers and cage cards, prepare necropsy reports and labels for biologic samples, archive samples in the tissue bank and ship biological samples to collaborators, maintain up to date records of culling and anesthesia logs.

25% Cellular and Molecular biology
With supervised oversight, prepare tissue lysates, isolate RNA and protein, quantitate nucleic acids and protein levels, perform qRT-PCR, PCR genotyping, nucleic acid and protein electrophoresis, prepare and refresh cell culture media. Establishing and maintaining mouse embryonic fibroblasts and culture of neuronal cell lines for functional assays.

All required qualifications must be included in the application materials.

Required Qualifications:

• BS/BA in a biomedical discipline, or a combination of related education and work experience to equal 4 years.
• Experience working with mice.
• Must have strong problem-solving skills, an ability to multitask and to work independently. 

Preferred Qualifications:

• Applicants should have an interest in health sciences and interest in working with laboratory animals.
• Experience with the Mouse Cloud database used to inventory mice and biological samples.
• Knowledge about DNA damage, DNA repair and genome instability disorders

About University of Minnesota Twin Cities

The University of Minnesota, founded in the belief that all people are enriched by understanding, is dedicated to the advancement of learning and the search for truth; to the sharing of this knowledge through education for a diverse community; and to the application of this knowledge to benefit the people of the state, the nation, and the world.

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